I thought I would make this blog post very meta by referring to another blog, written by Lior Pachter, which I think has something for many of us in it: http://liorpachter.wordpress.com (networks people, there’s a pretty scathing take-down of a quite well cited 2013 paper as one of the last posts – there seem to be a couple of posts labelled “network nonsense”!)

In particular I refer you to the list, that Lior Pachter has curated, which includes all variations of *-seq. You’ll see that practically all sequencing protocols take on this nomenclature of catchy descriptor + seq.

You will have heard mention of Ig-seq in talks by antibody people (with all Ig-seq experiments being curated in OAS by Alex). Ig-seq comes under the “Phenotyping” section of Lior’s list.

There’s also a few quirky theoretical applications of sequencing, for example a 2012 paper attempting to use sequencing for WIMP (https://en.wikipedia.org/wiki/Weakly_interacting_massive_particles) detection (which doesn’t seem to have taken off but it’s nice to see that the word has spread). There’s also BOINC-seq (Barcoding of Individual Neuronal Connections), which would use sequencing to reconstruct the neural connectome as an alternative to labour-intensive EM reconstruction (image segmentation algorithms have won out on this one, though).

These technologies either do underlie or could underlie a lot of our work – be this expression data for networks people (sequencing technologies which rely on differential chromatin or methylation states (e.g. FAIRE-seq and TAB-seq, respectively)), data on translation for the protein folders (Ribo-seq), or good old Ig-seq for antibody people such as myself.

I’m afraid small molecule and crystallography people are somewhat excluded from this sequencing party, but in any case I think it’s a cool blog!

All best wishes

Eve